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Handy Reference LSUHSC Clinical Services Guide
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Renal Tests
Kidney biopsies
- Native kidney biopsies are processed for light microscopy, immunofluorescent microscopy, and electron microscopy. Transplant biopsies may be handled similarly, or only light microscopy (with or without C4d stain) may be performed dependent upon the judgment of the clinician.
Light microscopy:
- Two-micron serial sections are cut.
- Duplicate slides stained with: Hematoxylin and eosin ,Periodic acid-Schiff, Jones methenamine silver, Trichrome stains.
- Unstained slides available for: BK polyomavirus, C4d, amyloid, or other immunohistochemical or histochemical stains as indicated.
Immunofluorescent microscopy:
- Four-micron sections are stained with: IgG, IgA, IgM, C3, C1q, kappa, lambda, and fibrinogen
Electron microscopy:
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One-micron thin sections are reviewed
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1-2 glomeruli are selected for ultrastructural examination.
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Tubulo-interstitial or vascular structures may be examined as indicated.
Surgical consult / specimen slides
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Submitted slides are reviewed.
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Wet tissue can be processed for routine histology
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Blocks can be submitted for recuts or immunohistochemical and histochemical stains as indicated
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